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快速高尔基染色试剂盒(神经元和胶质细胞)  DM1158

快速高尔基染色试剂盒(神经元和胶质细胞)产品货号:  DM1158 产品规格:  6 × 125ml/6 ×250ml产品简介:Golgi-Cox浸染法是研究神经元和胶质细胞正常和非正常形态*有效的方法之一。 使用Golgi技术,在药物处 理过的动物脑中和因神经疾病死亡的病人脑中发现了神经树突和树突微小的形态改变 。然而Golgi染色法的不可 靠性且费时已成为这种方法广泛应用的障碍。快速高尔基染色试剂盒(神经元和胶质细胞) 是根据Ramón-Moliner ,Glaser和Van der Loos所阐述的方法原 理设计的 。该试剂盒不仅极大地改进并简化了Golgi-Cox技术,而且被证实在显示神经元和胶质细胞,尤其是树 突棘的形态细节极为灵敏可靠。 生物生产的快速高尔基染色试剂盒已被广泛用于数种动物脑组织染色。 产品组成: 产品名称6 × 125ml6×250ml溶液A125ml250ml溶液B125ml250ml溶液C125ml×2250ml×2溶液D125ml250ml溶液E125ml250ml注:溶液C启封后请尽快用完。 需要但未包括在试剂盒里的物品1.      双蒸水或Milli-Q水2.      塑料/玻璃管或瓶 、琉璃样品回收器、天然毛画笔、滴瓶、塑料镊子3.      组织学耗材:明胶包被的显微镜载玻片盖玻片、染色罐乙醇二甲苯树胶封片剂光学显微镜 组织制备使用此试剂盒前必须仔细阅读以下说明!!l 所用容器(*好为塑料材质) 必须用蒸馏水冲洗干净。l 当溶液A和溶液B存在时不能使用金属器具。l 保持容器密封。l 用溶液A和溶液B处理的组织和切片,应该避光。l 除非特别指出, 以下流程需在室温下完成。  快速高尔基染色试剂盒(神经元和胶质细胞) 1.      杀死动物之前对实验动物进行深度麻醉。应尽快地从颅骨中取出动物的脑(或死去病人的脑),但操作时必 须非常小心以免损伤或压迫组织。注意l 除非完全必要,不要对动物进行灌注。如果确实需要对动物进行灌注(用4%的多聚甲醛处理不要超过5分钟), 一定不要对组织进行后固定处理。l 体积较大的脑部样本包括大鼠脑部应该用锋利的刀片切成大约10mm厚的块状。重要提示!2.      用双蒸水或Milli-Q水快速冲掉组织表面的血液。3.      把组织浸泡在由溶液A和B等体积混合的浸渍液中,室温下黑暗保存两周* 。浸泡6个小时以后或次日更换新 的浸渍液。*对于大多数情况浸泡2周是足够的。然而,不同的组织类型及组织的大小不同可能需要延长或缩短浸泡时间来达 到*佳效果。对于每种类型的组织的浸泡时间应该通过试验获得,但是对于大多数组织浸泡3周是足够的。注意, 延长浸泡时间可能增加染色背景。注意l 溶液A和溶液B的混合液至少在用之前24小时配制,并不能搅拌。l 采用以上方案则不会产生沉淀是关键的。l 制备好的浸泡液在用之前室温黑暗保存可长达一个月。l 每cm3 待研究组织至少用5ml浸泡液。注意用较少的浸泡液可能降低染色的灵敏性和可靠性。l 为取得*佳结果,在浸泡期间每周两次对装有组织的容器可轻轻地左右旋涡(一定不要晃动! )几秒钟。Warning溶液A和B(含有升汞,重铬酸钾和铬酸钾) 接触皮肤是有毒的,如果吞咽可能是致命的。实验应该在化学通风 橱中完成。当操作这些试剂时应穿戴防护服,手套和防护面具/眼镜。不要把溶液A和B的废弃物倒进水槽中!把溶液A和B的废弃物收集起来放到一个瓶子中,致电安全办公室或有执照的专业废物处理服务的部门处理些材 料。4.      转移组织到溶液C中室温黑暗至少72小时(可长达1周) 。24小时后或次日至少更换一次溶液。5.      在-20℃到-22℃的条件下用冷冻切片机将组织切成100-200um厚的薄片,也可用其它型号的显微镜薄片切片 机包括滑动切片机和振动切片机 。用样本回收器把样本转移到含有溶液C的明胶包被的显微镜载玻片上。载 玻片上多余的溶液用吸管吸走并用滤纸吸干(载玻片上的溶液必须尽可能地吸干否则切片将从载玻片上脱落 下来) 。切片可以室温下自然风干(不能用电风扇或电热板使其干燥) 。为取得*佳结果,应尽快地完成切 片,制备好的切片如果保存于载玻片盒中,室温黑暗条件下*长可保存3天。注意l 为避免组织受冷冻切片机的损害,并尽可能地保持细胞形态学,组织在进行冷冻切片之前应快速冰冻。 比如, 组织应按以下描述尽可能地快速冰冻:把组织放在一个塑料匙中慢慢地浸入含有干冰的异戊烷中预冷(为取 得*佳结果,温度应保持在-70℃以下并且浸入过程用时1分钟,越慢越好) 。组织完全浸入异戊烷之后,使 组织在异戊烷中浸几秒钟,然后再放置干冰上1分钟以确保组织能很好地冰冻。进行切片之前不要让组织融 化。l 切片机的型号可能会有不同,但所用的型号确保能切厚的切片(比如100um)。l 如果冰冻切片机只有一个温度设置,那么在进行切片之前把温度设置在-22℃至少4个小时。如果有两个温度 设置,那么设置槽内温度比样本温度低1℃ 。请注意大多数情况下-22℃是*佳的温度,然而,为了更顺利地 切出切片并没有破碎,不同型号的切片机和不同的组织可能需要更高或更低的槽内温度。l 对于用冰冻切片机切片, 以下几点提示是有益的: 1 )用蒸馏水把组织固定于样本盘上,但必须确保组织没 有融化(可以在干冰上完成) 。组织可以被固定在任何类型的组织冰冻介质上包括OCT ,但要避免切断介质。 不要在OCT中包埋组织,如果组织确实需要包埋,用TFM替代OCT 。2) 组织固定到样本盘后,放置组织于   快速高尔基染色试剂盒(神经元和胶质细胞) 干冰上10分钟,然后立即把固定有样本的样本盘安装到冰冻切片机上,等5分钟之后进行切片。3)切好的一 些切片(不要使用防卷板) ,如果组织温度过低比如切片显示出与刀片平行的裂缝,先等几分钟再进行下一 个切片,否则可继续切。l 浸泡的脑组织可用琼脂糖或明胶包埋然后用冰冻切片机切片。然后收集切片时(充满切片槽) ,必须使用溶 液C 。否则切片易干燥裂纹。l 如果用滑动切片机切浸泡的组织,样本盘和刀片都需维持在较低的温度(0℃以下) 按照操作手册的描述切 片固定在含有溶液C的载玻片上是重要的。VI.  染色步骤在染色过程中和盖盖玻片之前的任何一步都不要让切片变干1.      用双蒸水或Milli-Q水冲洗切片两次,每次4分钟。2.      把切片置于由1份溶液D , 1份溶液E和2份的双蒸水或Milli-Q水组成的混合液中10分钟。 比如:溶液D          10ml                                    溶液E        10ml                          双蒸水       20ml注意l 工作液*好现配现用,每100ml工作液*多用于100张切片(比如小鼠脑) , 根据切片的大小。l 盛有工作液的瓶子或染色罐必须盖好盖子防止试剂蒸发。l 为取得*佳结果,孵育期间应频繁搅拌工作液。3.      用蒸馏水或Milli-Q水冲洗切片两次,每次4分钟(蒸馏水应频繁更换新的)。4.      用结晶紫或硫堇对切片进行复染(可选步骤)。注意l 对于复染,延长步骤3的时间, 比如用冲洗4次每次5分钟代替原来的冲洗2次每次4分钟,或者更长的时间。5.     在50% ,75%和95%的乙醇中对切片进行脱水,每个浓度梯度脱水4分钟(不要跳过任何一个步骤)。6.      然后在无水乙醇中对切片进行脱水,4次,每次4分钟(不要延长时间)。7.      在二甲苯中透明,3次,每次4分钟,并且用树脂封片剂对盖玻片进行封片。注意l 盖玻片之前切片可以暂时存于二甲苯中几个小时。l 为取得*佳结果,*好用未稀释的封片剂。l 用Golgi染色的切片无论何时都应避光保存。 注意事项1.      快速高尔基染色试剂盒(神经元和胶质细胞)仅用于体外研究,不能用于诊断或其它应用。2.     试剂盒所包含有试剂是有毒的,吸入或接触皮肤是有害的,如果吞咽可能是致命的。不要用嘴吸。避免吸入 和接触皮肤和眼睛。如果接触,立即用大量的水冲洗并求助医生。3.      在化学通风橱下完成实验。操作这些试剂时须穿戴合适的防护服,手套和眼睛和面部防护罩,完成实验之后 把手彻底冲洗干净。 保存条件:  室温,有效期 12 个月。         东升国际-创意平台-注册畅享文化之梦!

人(Human)白细胞介素2(IL-2)ELISA检测试剂盒

本试剂盒只能用于科学研究,不得用于医学诊断人(Human)白细胞介素2(IL-2)ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被白细胞介素2(IL-2)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的白细胞介素2(IL-2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1.  血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2.  血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3.  细胞上清液:3000转离心10分钟去除颗粒和聚合物。4.  组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5.  保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1.  试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2.  实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3.  浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4.  严格按照说明书中标明的时间、加液量及顺序进行温育操作。5.  所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、7.5、15、30、60、120 pg/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1.  手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2.  自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1.  从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2.  设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3.  样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4.  除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5.  弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6.  每孔加入底物A、B各50μL,37℃避光孵育15min。7.  每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。试剂盒性能1.  准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2.  灵敏度:*低检测浓度小于1.0 pg/mL。3.  特异性:不与其它可溶性结构类似物交叉反应。4.  重复性:板内、板间变异系数均小于15%。5.  贮藏:2-8℃,避光防潮保存。6.  有效期:6个月免责声明1.   试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2.   严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。  FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interleukin 2 (IL-2) ELISA Kit instruction Intended useThis IL-2 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-2 in the sample, this IL-2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-2 concentration. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note:  The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1.  Standard microplate reader(450nm)2.  Precision pipettes and Disposable pipette tips.3.  37 ℃ incubatorPrecautions1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3.  Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,7.5,15,30,60,120 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage:  2-8℃.validity: six months.  FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

¥1680
人(Human)γ干扰素(IFN-γ)ELISA检测试剂盒

本试剂盒只能用于科学研究,不得用于医学诊断人(Human)γ干扰素(IFN-γ)ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被γ干扰素(IFN-γ)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的γ干扰素(IFN-γ)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1.  血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2.  血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3.  细胞上清液:3000转离心10分钟去除颗粒和聚合物。4.  组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5.  保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1.  试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2.  实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3.  浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4.  严格按照说明书中标明的时间、加液量及顺序进行温育操作。5.  所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、50、100、200、400、800 pg/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1.  手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2.  自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1.  从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2.  设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3.  样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4.  除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5.  弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6.  每孔加入底物A、B各50μL,37℃避光孵育15min。7.  每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1.  准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2.  灵敏度:*低检测浓度小于1.0 pg/mL。3.  特异性:不与其它可溶性结构类似物交叉反应。4.  重复性:板内、板间变异系数均小于15%。5.  贮藏:2-8℃,避光防潮保存。6.  有效期:6个月免责声明1.   试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2.   严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。  FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interferon γ (IFN-γ) ELISA Kit instruction Intended useThis IFN-γ ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-γ ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note:  The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1.  Standard microplate reader(450nm)2.  Precision pipettes and Disposable pipette tips.3.  37 ℃ incubatorPrecautions1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3.  Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,50,100,200,400,800 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage:  2-8℃.validity: six months.  FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

¥1680
CD68 Monoclonal Antibody(6F3)

Catalog NoIsotypeReactivityApplicationsGene Name Protein NameImmunogen Specificity FormulationSource PurificationDilution Concentration PurityStorage Stability Synonyms Observed Band Cell PathwayTissue SpecificityDM-Ab-13828IgG Human;Mouse;Rat IHC;IFCD68MacrosialinSynthetic Peptide of CD68The antibody detects endogenous CD68 proteins.PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Monoclonal, MouseThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.WB 500-2000 1:200 IF 1:50-200 1 mg/ml≥90%-20°C/1 yearCD68; Macrosialin; Gp110; CD6837kD[IsoformShort]: Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor celllines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.                  

¥1800
EID2B antibody  DM02652

EID2B antibody Product Information Catalog No. : DM02652 Size: 100µg Form: liquid Purification: Immunogen affinity purified Purity: ≥95% as determined by SDS-PAGEHost: Rabbit Clonality: polyclonal Clone ID: None IsoType: IgGStorage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) Background       Acts as a repressor of MYOD-dependent transcription, glucocorticoid receptor-dependent transcription, and muscle differentiation.Immunogen information      Immunogen: EP300 interacting inhibitor of differentiation 2B Synonyms: EP300-interacting inhibitor of differentiation 2B (EID-2B)|EID-2-like inhibitor of differentiation 3 (EID-3)|EID2B|EID3 Observed MW: 43 kDa Uniprot ID : Q96D98Application       Reactivity: Human Tested Application: ELISA, WB Recommended dilution:WB: 1:500-1:2000

¥398
​ATF-3 Polyclonal Antibody

ATF-3 Polyclonal Antibody Catalog No DM-Ab-01554 Isotype IgG Reactivity Human;Mouse;Rat Applications WB;IHC;IF;ELISA Gene Name ATF3Protein Name Cyclic AMP-dependent transcription factor ATF-3 Immunogen The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180 Specificity ATF-3 Polyclonal Antibody detects endogenous levels of ATF-3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 yearSynonyms ATF3; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 Observed Band 21kD Cell Pathway Nucleus . Tissue Specificity Amygdala,Brain,Cerebellum,Muscle,Function function:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,Background activating transcription factor 3(ATF3) Homo sapiens This gene encodes a possible that alternative splicing of this gene may be physiologically important in matters needing attention Avoid repeated freezing and thawing!Usage suggestions This product can be used in immunological reaction related experiments. For Western Blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). Western blot analysis of lysates from RAW264.7 cells, using ATF3 Antibody. The lane on the right is blocked with the synthesized peptide. member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is more information, please consult technical personnel. the regulation of target genes. [provided by RefSeq, Apr 2011],Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). 

¥1980
香菇多糖 10g

香菇多糖       香菇多糖(Lentinan,简称LNT)是一种从优质香菇子实体中提取的有效活性成分,也是香菇的主要有效成分,它属于多糖类物质。中文名称:香菇多糖中文别名:香菇多糖;瘤停能;香菇糖;Lentinan 香菇多糖;香菇粉;香菇提取物;云芝多糖;猪苓多糖;香菇菌多糖英文名称:Lentinan英文别名:Lentinan;LC 33;biomoduline;LENTINAN (SHIITAKE MUSHROOM POLYSACCHARIDES);LENTINEX(R);lentinan vial;beta-D-glucopyranosyl-(1->6)-[beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->6)]-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->3)]-beta-D-glucopyranose;Bromoduline;DRG-0171;LC-33;Lentinan polysaccharide;Polysacharid lentinus edodes;β(1-3) linked D-glucose with 2 side chains of single β(1-6) D-glucose every five unit.;BiomodulineCAS号:37339-90-5分子式:C42H72O36分子量:1153.00香菇多糖英文名:LentinanCAS号:37339-90-5纯度:BR,50%沸点: 1472°Cat760mmHg比旋光度: D20 +13.5-14.5° (in 2% NaOH); +19.5-21.5° (in 10% NaOH)溶解性: 溶于碱溶液或甲酸,微溶于热水或二甲亚砜,不溶于冷水、醇、乙醚、氯仿、吡啶或六甲基磷酰胺。储存条件: RT化学组成与性质化学组成:香菇多糖主要以β-D(1→3)葡聚糖残基为主链,侧链为(1→6)葡聚糖残基的葡聚糖。其活性成分是具有分支的β—(1—3)—D—葡聚糖,主链由β—(1—3)—连接的葡萄糖基组成,沿主链随机分布着由β—(1—6)连接的葡萄糖基,呈梳状结构。性质:香菇多糖为灰白色粉末,大多为酸性多糖,溶于水、稀碱,尤其易溶于热水,不溶于乙醇、丙酮、乙酸乙酯、乙醚等有机溶剂。其水溶液呈透明黏稠状。功效与作用抗病毒:香菇多糖能够提高感染细胞的免疫力,增强细胞膜的稳定性,抑制细胞病变,促进细胞修复,从而起到抗病毒的作用。它可以用于乙型肝炎和艾滋病等病毒性疾病的治疗。抗肿瘤:香菇多糖是一种宿主免疫增强剂,虽然它本身在体内无直接杀伤肿瘤细胞的作用,但可以通过增强机体的免疫功能而发挥抗肿瘤活性。它能增强脾脏和腹腔的NK细胞活性,诱生干扰素,与白细胞介素类或干扰素诱导剂有协同作用。香菇多糖主要用于不能手术或复发性胃癌、肝癌、膀胱癌等肿瘤患者的辅助治疗,能够缓解症状,提高病人免疫功能,纠正微量元素失调。调节免疫功能:香菇多糖能够识别脾脏及肝脏中抗原的巨噬细胞,促进淋巴细胞活化因子的产生,释放各种辅助性T细胞因子,增强宿主腹腔巨噬细胞吞噬率,从而调节机体的免疫功能。其他作用:香菇多糖还具有降血压、降血脂等生理功能,并可用于治疗具有抗药性的肺结核和老年性慢性支气管炎等疾病。提取与纯化       香菇多糖的提取多采用热水及稀碱溶液,避免在强酸、碱溶液中进行,以防止多糖中糖苷键断裂及构象变化。常用的分离纯化方法包括沸水浸提、乙醇沉淀、透析及柱层析等步骤。

¥220
产品名称

产品描述

Stat3 Polyclonal Antibody

Stat3 Polyclonal AntibodyCatalog No DM-Ab-02054Isotype IgGReactivity Human;Mouse;RatApplications IF;WB;IHC;ELISAGene Name STAT3Protein Name Signal transducer and activator of transcription 3Immunogen The antiserum was produced against synthesized peptide derived from humanSTAT3. AA range:672-721Specificity Stat3 Polyclonal Antibody detects endogenous levels of Stat3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen. Dilution IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Concentration 1 mg/mlPurity ≥90%Storage Stability -20°C/1 yearSynonyms STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phaseresponse factorObserved Band 88kDCell Pathway Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Function     disease:Defects in STAT3 are the cause of hyperimmunoglobulin E recurrentELISA: 1/5000. Not yet tested in other applications.Translocated into the nucleus upon tyrosine phosphorylation and dimerization, inresponse to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells(PubMed:31899195). Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitoryfactor (LIF) stimulation, accumulates in the nucleus. The complex composed ofBART and ARL2 plays an important role in the nuclear translocation and retentioninfection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known ashyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunityand connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctivecoarse facial appearance, abnormal dentition, hyperextensibility of the joints, andbone fractures.,function:Transcription factor that binds to the interleukin-6of STAT3. Identified in a complex with LYN and PAG1.Jiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derivedmesenchymal stem cell secretome promotes hepaticregeneration in miniature pigs after liverischaemia-reperfusion combined with partialresection. Stem Cell Res Ther 12, 218 (2021).Liu, Bowen, et al. "Oncoprotein HBXIP enhancesHOXB13 acetylation and co-activates HOXB13 toconfer tamoxifen resistance in breast cancer." Journalof hematology & oncology 11.1 (2018): 26.Immunofluorescence analysis of Hela cell. 1,Stat3Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.was diluted at 1:200(4° overnight). 2, Goat Anti RabbitAlexa Fluor 594 Catalog:RS3611 was diluted at1:1000(room temperature, 50min). Goat Anti MouseAlexa Fluor 488 Catalog:RS3208 was diluted at1:1000(room temperature, 50min).

¥1800
IL-6 Polyclonal Antibody

IL-6 Polyclonal AntibodyCatalog No  DM-Ab-16022Isotype         IgGReactivity         Human;Rat;MouseApplications         WB;IHC;IF;ELISAGene Name IL6Protein Name Interleukin-6Immunogen The antiserum was produced against synthesized peptide derived from theInternal region of human IL6. AA range:131-180Specificity IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen. Dilution Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300Concentration 1 mg/mlPurity ≥90%Storage Stability -20°C/1 yearSynonyms IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTLdifferentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2Observed Band 23kDCell Pathway Secreted . Tissue Specificity Produced by skeletal muscle. Function disease:At least 1 polymorphism in the IL6 gene renders HIV-infected menNot yet tested in other applications.susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma ofspindle cells mixed with angiomatous tissue. A relatively rare malignant skintumor that results in multifocal purplish coloured papules or plaques thateventually form nodules. Most commonly seen in patients who suffer fromAIDS.,disease:Genetic variations in IL6 are associated with susceptibility tosystemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenilerheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despitemedical treatment, many children still experience early joint destruction, necessitating surgical replacement.,function:IL-6 is a cytokine with a wide varietyof biological functions: it plays an essential role in theJiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derivedmesenchymal stem cell secretome promotes hepaticregeneration in miniature pigs after liverischaemia-reperfusion combined with partialresection. Stem Cell Res Ther 12, 218 (2021)Liu, Bowen, et al. "Oncoprotein HBXIP enhancesHOXB13 acetylation and co-activates HOXB13 toconfer tamoxifen resistance in breast cancer." Journalof hematology & oncology 11.1 (2018): 26.Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.Immunofluorescence analysis of Hela cell. 1,Stat3Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.was diluted at 1:200(4° overnight). 2, Goat Anti RabbitAlexa Fluor 594 Catalog:RS3611 was diluted at1:1000(room temperature, 50min). Goat Anti MouseAlexa Fluor 488 Catalog:RS3208 was diluted at1:1000(room temperature, 50min).

¥1800
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